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| Registros recuperados: 50 | |
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| Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine. |
| In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were... |
| Tipo: Text |
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf |
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| Saulnier, Denis; De Decker, Sophie; Haffner, Philippe. |
| Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of... |
| Tipo: Text |
Palavras-chave: V. aestuarianus; Vibrio; Taqman; Real time PCR; Pathogen; Oyster; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6447.pdf |
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| Goarant, Cyrille; Ansquer, Dominique; Herlin, Jose; Domalain, David; Imbert, Frederic; De Decker, Sophie. |
| The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but... |
| Tipo: Text |
Palavras-chave: Epidemiology; Vibrio; Diseases and their control; Shrimp. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1110.pdf |
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| Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine. |
| In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was... |
| Tipo: Text |
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf |
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| Cantet, Franck; Hervio-heath, Dominique; Caro, A; Le Mennec, Cecile; Monteil, Caroline; Quemere, Catherine; Jolivet-gougeon, Anne; Colwell, Rita R.; Monfort, Patrick. |
| Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 x 10.3 MPN/l, 0.09 to 1.1 x 10.3 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were... |
| Tipo: Text |
Palavras-chave: Vibrio; Lagoons; Shellfish; Water; Sediment; Human pathogen. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25890/23889.pdf |
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| De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis. |
| Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and... |
| Tipo: Text |
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf |
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| Falaise, Charlotte; James, Adele; Travers, Marie-agnes; Zanella, Marie; Badawi, Myriam; Mouget, Jean-luc. |
| Marennine, the water-soluble blue pigment produced by the marine diatom Haslea ostrearia, is known to display antibacterial activities. Previous studies have demonstrated a prophylactic effect of marennine on bivalve larvae challenged with a pathogenic Vibrio splendidus, suggesting that the blue Haslea is a good candidate for applications in aquaculture as a source of a natural antimicrobial agent. Indeed, the genus Vibrio is ubiquitous in aquaculture ecosystems, and regular events of pathogenic invasion cause some of the biggest losses worldwide. To better characterize the effects of marennine on Vibrios, a panel of 30 Vibrio strains belonging to 10 different species was tested, including bivalve pathogenic species (e.g., Vibrio crassostreae and Vibrio... |
| Tipo: Text |
Palavras-chave: Antibacterial activity; Diauxie; Haslea; Hormesis; Marennine; Vibrio. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00484/59598/62602.pdf |
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| Cochennec, Nathalie; Haffner, Philippe; Levy, Peva; Saulnier, Denis; Fougerouse, A.; Langy, Sandra. |
| In French Polynesia, black pearl cultivation now takes place on about 30 of the 118 atolls or islands. This activity has largely replaced copra, coconut oil and fishing as a source of income. Around 1,000 pearl farms, generally small ones of less than 5 ha, are registered. The pearl farms are mainly owned by families. In 1998, about 7,000 persons were employed in theentire pearl industry, from production to retailing. In value, this activity represents the first exportation of French Polynesian products. From 1988 to date, exports increased exponentially before leveling. Starting with less than two kilograms in 1972, exports have now stabilized at Il tons of "black" pearls. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiology; Grafting process; Pinctada margaritifera. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4928.PDF |
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| Faury, Nicole; Saulnier, Denis; Thompson, F; Gay, Melanie; Swings, J; Leroux, Frederique. |
| Polyphasic analysis of five new Vibrio isolates originating from the haernolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T) =LMG 22240(T)=Clp 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine... |
| Tipo: Text |
Palavras-chave: Identification; Vibrio; Crasssotrea gigas; Ostreiculture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-591.pdf |
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| Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel. |
| Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La.... |
| Tipo: Text |
Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf |
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| Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine. |
| Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French... |
| Tipo: Text |
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf |
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| Robb, F; Muir, D; Davis, C. |
| Some mechanisms of selection which operate on marine Vibrio and Pseudomonas strains during survival in dilute environments have been examined. Mannitol uptake and utilisation systems are more efficient in the Vibrio strain compared with the Pseudomonas strain during starvation survival. A Vibrio mutant strain lacking mannitol uptake showed no greater loss of viability during starvation survival than mannitol positive strains. The Vibrio strain appears to be constitutive with respect to mannitol uptake systems, whereas the Pseudomonas strain examined is inducible. Active uptake systems for glucose, glutamate, alanine and aspartic acid were efficiently maintained during starvation survival in the Vibrio strain. Results suggest that membrane uptake systems... |
| Tipo: Text |
Palavras-chave: Pseudomonas; Vibrio; Bacteria; Physiology; Alcohols; Food conversion; Survival; Starvation; Metabolism. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-960.pdf |
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| Bruni, V; Crisafi, E; Maugeri, T; Zaccone, R. |
| 110 Vibrio strains were isolated, at various times of the year, from samples of sea-water supply, breeding-tank water and discarded fish used as food at the breeding stations. From the results obtained by the biochemical and cultural tests, it was possible to identify most of the isolated strains as Vibrio anguillarum . Strains with intermediate characteristics, above-all positive saccharose, proved to be numerous. The authors discuss the occurrence, in particular environments such as those being studied, of atypical or intermediate vibrio strains which are difficult to classify. |
| Tipo: Text |
Palavras-chave: Vibrio; Microbiological culture; Bacteria; Food; Minced products; Fish ponds; Sea water; Identification; Marine aquaculture; Bacteria collecting devices; Microbiological analysis. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-997.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Rouze, Heloise; Lecellier, Gael; Saulnier, Denis; Berteaux-lecellier, Veronique. |
| Coral disease outbreaks have increased over the last three decades, but their causal agents remain mostly unclear (e.g., bacteria, viruses, fungi, protists). This study details a 14-month-long survey of coral colonies in which observations of the development of disease was observed in nearly half of the sampled colonies. A bimonthly qPCR method was used to quantitatively and qualitatively evaluate Symbiodinium assemblages of tagged colonies, and to detect the presence of Vibrio spp. Firstly, our data showed that predisposition to disease development in general, and, more specifically, infection by Vibrio spp. in Acropora cytherea depended on which clades of Symbiodinium were harbored. In both cases, harboring clade D rather than A was beneficial to the... |
| Tipo: Text |
Palavras-chave: Coral disease; Dynamic; Resistance; Symbiodinium assemblages; Vibrio. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00307/41848/41080.pdf |
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| De Decker, Sophie. |
| The bacteria of the Vibrio genus are one of the most abundant groups in the aquatic ecosystems. Two species of vibrios, Vibrio splendidus and V. Aestuarianus, have been associated, in some cases, to mortality in flat oyster Crassostrea gigas farms, most of the time during the summer period. This doctoral thesis studies the Vibrio-host interaction and their modulations according to the host's pathogenic virulence and its genetic, physiologic and immunitary parameters. These factors will be characterised by in vitro and in vivo approaches (experimental infection). The characterization of contrasted genetic families according to their sensitivity to these vibrios will enable the implementation of new improvement strategies in oyster farms (prophylactic... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres génétiques; Virulence; Oyster; Crassostrea gigas; Pathogène; Vibrio. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3431.pdf |
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| Registros recuperados: 50 | |
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